Working group Seifert
The Seifert research group explores the influence of cellular and molecular mediators of an immune response on endothelial cell properties and function to better understand the pathogenesis of diseases.
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Funding
- Friede Springer Herzstiftung (2015- 2018)
- DFG Project 657/12-1 (2015-2019)
- Einstein Center Regenerative Medicine (ECRT), grant (2017-2019)
- EU Project PACE (2016-2021)
Techniques & services
- Primary human cell cultures (endothelial cells, mesenchymal stromal cells, cardiac cells, tenocytes)
- Isolation of extracellular vesicles (EVs) by differential ultracentrifugation, characterization (EM, flow cytometry, protein/BCA, NTA)
- Immune cell isolation, separation and co-culture assays
- Flow cytometry analysis
- Fluorescence microscopy/ High Content Screening
- Proliferation assays
- Cytotoxicity assays
- Migration assays
- Apoptosis Assays
- Monocyte/Macrophages (differentiation/polarization assay)
- Endothelial assays (activation, angiogenesis)
- ELISA & Multiplex assays
- Quantitative real time RT-PCR
- Immune histology