Working group Seifert
The research group conducts research in the field of cell and tissue transplantation and investigates the mechanisms of immunological recognition and modulation.
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Monitoring and Modulation of Immune Responses to Cell and Tissue Transplants
“We aim to unravel the complex molecular language between regenerative cells or their derivatives with the body´s main players of immune responses, which will ultimately enable safe and effective therapies and promote the discovery of new therapeutic avenues.”
Funding
- Friede Springer Herzstiftung (2015- 2018)
- DFG Project 657/12-1 (2015-2019)
- EU Project PACE (2016-2021)
- Einstein Center Regenerative Medicine (ECRT), grant (2017-2019)
Techniques & services
- Primary human cell cultures (endothelial cells, mesenchymal stromal cells, cardiac cells, tenocytes)
- Isolation of extracellular vesicles (EVs) by differential ultracentrifugation, characterization (EM, flow cytometry, protein/BCA, NTA)
- Immune cell isolation, separation and co-culture assays
- Flow cytometry analysis
- Fluorescence microscopy/ High Content Screening
- Proliferation assays
- Cytotoxicity assays
- Immune cell migration
- Apoptosis Assays
- Monocyte/Macrophage/Dendritic cell differentiation/polarization assay
- Endothelial assays (e.g. activation, angiogenesis)
- Cytokine/Chemokine/Growth factor detection assays (ELISA & Multiplex assays)
- Quantitative real time RT-PCR (mRNA; miRNA)
- Nanostring assays (human Myeloid Panel, human miRNA Panel)
- Immune histology
- Raster electron microscopy (REM)
- Humanized mouse models