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Working group Seifert

The research group conducts research in the field of cell and tissue transplantation and investigates the mechanisms of immunological recognition and modulation.
 

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Monitoring and Modulation of Immune Responses to Cell and Tissue Transplants

“We aim to unravel the complex molecular language between regenerative cells or their derivatives with the body´s main players of immune responses, which will ultimately enable safe and effective therapies and promote the discovery of new therapeutic avenues.”

Funding

  • Friede Springer Herzstiftung (2015- 2018)
  • DFG Project 657/12-1 (2015-2019)
  • EU Project PACE (2016-2021)
  • Einstein Center Regenerative Medicine (ECRT), grant (2017-2019)

Techniques & services

  • Primary human cell cultures (endothelial cells, mesenchymal stromal cells, cardiac cells, tenocytes)
  • Isolation of extracellular vesicles (EVs) by differential ultracentrifugation, characterization (EM, flow cytometry, protein/BCA, NTA)
  • Immune cell isolation, separation and co-culture assays
  • Flow cytometry analysis
  • Fluorescence microscopy/ High Content Screening
  • Proliferation assays
  • Cytotoxicity assays
  • Immune cell migration
  • Apoptosis Assays
  • Monocyte/Macrophage/Dendritic cell differentiation/polarization assay
  • Endothelial assays (e.g. activation, angiogenesis)
  • Cytokine/Chemokine/Growth factor detection assays (ELISA & Multiplex assays)
  • Quantitative real time RT-PCR (mRNA; miRNA)
  • Nanostring assays (human Myeloid Panel, human miRNA Panel)
  • Immune histology
  • Raster electron microscopy (REM)
  • Humanized mouse models

Contact details

Prof. Martina Seifert

t: +49 30 450 539 435
f: +49 30 450 539 933